Kinetics of Salmonella typhimurium binding with antibody by immunoassays using a surface plasmon resonance biosensor

Kyong-Hwan Chung; Hyun-Soo Hwang; Ki-Young Lee

Journal of Industrial and Engineering Chemistry, Vol.16, No.1, 115-118, January, 2010

DOI10.1016/j.jiec.2010.01.009 Export Citation

Kinetics of Salmonella typhimurium binding with antibody by immunoassays using a surface plasmon resonance biosensor

Kyong-Hwan Chung, Hyun-Soo Hwang¹, and Ki-Young Lee^{2, †}

Center for Functional Nano Fine Chemicals & Department of Applied Chemical Engineering, Chonnam National University, Gwangju 500-757, Republic of Korea
¹Department of Material and Biochemical Engineering, Chonnam National University, Gwangju 500-757, Republic of Korea
²Department of Applied Chemical Engineering & The Research Institute for Catalysis, Chonnam National University, Gwangju 500-757, Republic of Korea

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This study examined the kinetics of Salmonella typhimurium binding to an antibody using a direct assay and sandwich assay with a surface plasmon resonance (SPR) biosensor. A kinetic model was proposed based on curve-fitting of the experimental SPR sensorgrams using an integral rate equation. The SPR sensitivity in the sandwich assay was significantly higher than that in the direct assay. The association rate constants and affinities of the cell in the sandwich assay were double those in the direct assay. The enhanced binding attraction between the cell and detection antibody in the sandwich assay improved the lower limit of detection.

Keywords:Kinetics;Immunoassays;Salmonella typhimurium;Surface plasmon resonance;Antibody

[References]

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Kim HJ, Jeong YS, Lee YS, J. Ind. Eng. Chem., 5(1), 69 (1999)
Park HW, Kim JD, J. Ind. Eng. Chem., 15(4), 578 (2009)
Kim YK, Kim HJ, J. Ind. Eng. Chem., 15(2), 229 (2009)
Oh BK, Kim YK, Bae YM, Lee WH, J. Microbiol. Biotechnol., 12, 780 (2002)
Min JH, Baeumner A, J. Ind. Eng. Chem., 9(1), 1 (2003)
Fagerstram LG, Karlsson R, in: Scheller F, Schmid RD (Eds.), Biosensor techniques : Fundamentals, Techniques, Applications, GBF Monographs, vol. 17, VCH Publishers, Cambridge, England, 1991, p. 949.
Fagerstram LG, in: Villafranca JJ (Ed.), Techniques in Protein Chemistry, vol. II, Academic Press, New York, USA, 1991, p. 65.
Lundstrom I, Biosens. Bioelectron., 9, 725 (1994)
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Handa H, Gurczynski S, Jackson MP, Auner G, Walker J, Mao G, Surf. Sci., 602, 1392 (2008)
Karlsson R, Michaelsson A, Mattersson L, J. Immunol. Methods, 145, 229 (1991)
Altschuh D, Dubs MC, Weiss E, Zeder-Lutz G, van Regemmortel MHV, Biochemistry, 31, 6298 (1992)
Wohlhueter RM, Parekh K, Udhayakumar V, Fang S, Lal AA, J. Immunol., 153, 181 (1994)
Chung KH, Yang KS, Kim J, Kim JC, Lee KY, J. Microbiol. Biotechnol., 17, 1848 (2007)
Chung KH, Park JS, Hwang HS, Kim JC, Lee KY, J. Microbiol. Biotechnol., 17, 1191 (2007)
Medina MB, Fratamico PM, Biotechnol. Tech., 12, 235 (1988)
Holmes SD, May K, Johansson V, Markey F, Critchley LA, J. Microbiol. Methods, 28, 77 (1997)
Medina MB, Int. J. Food Microbiol., 69, 199 (2001)
Myszka DG, Rich RL, Pharm. Sci. Technol. Today, 3, 310 (2000)
Li Y, Ren J, Nakajima H, Kim BK, Soh N, Nakano K, Imato T, Talanta, 77, 473 (2008)

[Referenced By]

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[1] Schiffman G, Rev. Infect. Dis., 3, 224 (1981)

[2] Lofas S, Sens. Actuators B, 5, 79 (1991)

[3] Kim HJ, Jeong YS, Lee YS, J. Ind. Eng. Chem., 5(1), 69 (1999)

[4] Park HW, Kim JD, J. Ind. Eng. Chem., 15(4), 578 (2009)

[5] Kim YK, Kim HJ, J. Ind. Eng. Chem., 15(2), 229 (2009)

[6] Oh BK, Kim YK, Bae YM, Lee WH, J. Microbiol. Biotechnol., 12, 780 (2002)

[7] Min JH, Baeumner A, J. Ind. Eng. Chem., 9(1), 1 (2003)

[8] Fagerstram LG, Karlsson R, in: Scheller F, Schmid RD (Eds.), Biosensor techniques : Fundamentals, Techniques, Applications, GBF Monographs, vol. 17, VCH Publishers, Cambridge, England, 1991, p. 949.

[9] Fagerstram LG, in: Villafranca JJ (Ed.), Techniques in Protein Chemistry, vol. II, Academic Press, New York, USA, 1991, p. 65.

[10] Lundstrom I, Biosens. Bioelectron., 9, 725 (1994)

[11] Homola J, Yee S, Myszk D, in: Ligler FS, Taitt CAR (Eds.), Optical Biosensors-Present and Future, Elsevier Science B.V., Amsterdam, 2002, p. 207.

[12] Handa H, Gurczynski S, Jackson MP, Auner G, Walker J, Mao G, Surf. Sci., 602, 1392 (2008)

[13] Karlsson R, Michaelsson A, Mattersson L, J. Immunol. Methods, 145, 229 (1991)

[14] Altschuh D, Dubs MC, Weiss E, Zeder-Lutz G, van Regemmortel MHV, Biochemistry, 31, 6298 (1992)

[15] Wohlhueter RM, Parekh K, Udhayakumar V, Fang S, Lal AA, J. Immunol., 153, 181 (1994)

[16] Chung KH, Yang KS, Kim J, Kim JC, Lee KY, J. Microbiol. Biotechnol., 17, 1848 (2007)

[17] Chung KH, Park JS, Hwang HS, Kim JC, Lee KY, J. Microbiol. Biotechnol., 17, 1191 (2007)

[18] Medina MB, Fratamico PM, Biotechnol. Tech., 12, 235 (1988)

[19] Holmes SD, May K, Johansson V, Markey F, Critchley LA, J. Microbiol. Methods, 28, 77 (1997)

[20] Medina MB, Int. J. Food Microbiol., 69, 199 (2001)

[21] Myszka DG, Rich RL, Pharm. Sci. Technol. Today, 3, 310 (2000)

[22] Li Y, Ren J, Nakajima H, Kim BK, Soh N, Nakano K, Imato T, Talanta, 77, 473 (2008)