Korean Journal of Chemical Engineering, Vol.27, No.3, 919-924, March, 2010
Optimization of the fermentation conditions and partial characterization for acido-thermophilic α-amylase from Aspergillus niger NCIM 548
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A high performance thermostable α-amylase at low pH values has been synthesized. Sugarcane bagasse was hydrolyzed in a dilute acid solution and utilized as carbon source for the growth of Aspergillus niger strain NCIM 548. Glucose, xylose and arabinose with the ratio of 1.0 : 0.9 : 0.3 (w/w/w) were detected in the hydrolyzate by HPLC analysis. Optimization of the fermentation conditions for α-amylase production was performed by varying four influential parameters such as Sugarcane bagasse hydrolyzate (SBH), NH4Cl, pH and incubation time using a central composite design (CCD) under response surface methodology (RSM). The optimum values of SBH, NH4Cl, pH and incubation time were 20.49, 2.34 g/l, 5.65 and 76.67 h, respectively. The acido-thermophilic enzyme showed maximum stability at 70 ℃ and pH value of 4. The rate constant, K(m) and maximum reaction rate, V(max) were 18.79 g/l and 15.85 g/l·min, respectively.
Keywords:α-Amylase;Aspergillus niger;Response Surface Methodology;Sugarcane Bagasse Hydrolyzate;Optimization
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