Melanogenesis inhibitory effect of dehydroevodiamine isolated from fruits of Evodia rutaecarpa

Lian Hua Luo; Jeong-Woo Seo; Dung Hoang Nguyen; Eun-Ki Kim; Soon Ah Kang; Dae-Hyuk Kim; Chul Ho Kim

Korean Journal of Chemical Engineering, Vol.27, No.3, 915-918, March, 2010

DOI10.1007/s11814-010-0147-1 Export Citation

Melanogenesis inhibitory effect of dehydroevodiamine isolated from fruits of Evodia rutaecarpa

Lian Hua Luo^{1, 2}, Jeong-Woo Seo¹, Dung Hoang Nguyen³, Eun-Ki Kim³, Soon Ah Kang⁴, Dae-Hyuk Kim², and Chul Ho Kim^{1, †}

¹Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongup, Korea
²Institute for Molecular Biology and Genetics, Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Korea
³Department of Biological Engineering, National Lab of Skin Bioactive Material, Inha University, Incheon, Korea
⁴Department of Fermented Food Science, Seoul University of Venture and Information, Seoul, Korea

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Dehydroevodiamine, an alkaloid, was isolated from the fruit of Evodia rutaecarpa and melanin production, and tyrosinase inhibition in B16F10 melanoma cells treated with the isolated dehydroevodiamine was investigated. The compound decreased melanin synthesis significantly without promoting cytotoxicity. The IC50 value of dehydroevodiamine for melanogenesis and cell viability were 59.8 μM and 90.0 μM, respectively. The L-dopa oxidase activity of mushroom tyrosinase was reduced after dehydroevodiamine treatment by about 22.4% at a concentration of 33.2 μM. However, there was no effect on cellular tyrosinase activity. These results indicate that the observed decrease in melanin content after treatment with dehydroevodiamine was attributed to the direct inhibition of tyrosinase activity, rather than the suppression of tyrosinase gene expression. Dehydroevodiamine may be a promising new agent for use in cosmeceutical application.

Keywords:Melanogenesis;Evodia rutaecarpa;Dehydroevodiamine;Skin Whitening;Cosmeceutical

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