Chondrocyte 3D-Culture in RGD-Modified Crosslinked Hydrogel with Temperature-Controllable Modulus

Hyesun Lee; Bo Gyu Choi; Hyo Jung Moon; Jiyeon Choi; Kwideok Park; Byeongmoon Jeong; Dong Keun Han

Macromolecular Research, Vol.20, No.1, 106-111, January, 2012

DOI10.1007/s13233-012-0074-6 Export Citation

Chondrocyte 3D-Culture in RGD-Modified Crosslinked Hydrogel with Temperature-Controllable Modulus

Hyesun Lee^{1, 2}, Bo Gyu Choi², Hyo Jung Moon², Jiyeon Choi^{1, 2}, Kwideok Park^{1, 2}, Byeongmoon Jeong^{2, †}, and Dong Keun Han^{1, 2}

¹Center for Biomaterials, Korea Institute of Science and Technology, Seoul, 130-650, Korea
²Department of Bioinspired Science (WCU), Department of Chemistry and Nano Science, Ewha Womans University, Seoul, 120-750, Korea

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Based on the thermosensitivity of Pluronic® F127 and the cytointeractability of Arg-Gly-Asp (RGD) sequences, RGD-modified F127 dimethacrylate (FM-RGD) hydrogel was investigated as a 3-dimensional culture matrix for chondrocytes. Chondrocytes were encapsulated in the hydrogel by radical copolymerization of FM-RGD and FM in the presence of cells. The FM-RGD hydrogel modulus could be modulated by temperature variation from 15℃ (1,300 Pa) to the culture temperature of 37 ℃ (8,900 Pa), at which the hydrogel provided a condrocyte-compatible microenvironment. Compared with the hydrogel prepared from F127 dimethacrylate (FM) without RGD, the RGD-modified hydrogel produced significant (p<0.01) improvements in cell proliferation, DNA production, and viability while allowing the chondrocytes to maintain their original spherical phenotypes.

Keywords:stimuli-sensitive polymers;biomimetic;modulus;hydrogel;biomaterial.

[References]

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[Referenced By]

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[2] Atala A, Curr. Opin. Biotechnol., 20, 575 (2009)

[3] Dvir T, Timko BP, Kohane DS, Langer R, Nat. Nanotechnol., 6, 13 (2011)

[4] Dutta RC, Dutta AK, Biotechnol. Adv., 27, 334 (2009)

[5] Tibbitt MW, Anseth KS, Biotechnol. Bioeng., 103(4), 655 (2009)

[6] Zhu J, Biomaterials., 31, 4639 (2010)

[7] Schmedlen RH, Masters KS, West JL, Biomaterials., 23, 4325 (2002)

[8] Lee KY, Mooney DJ, Chem. Rev., 101(7), 1869 (2001)

[9] Hersel U, Dahmen C, Kessler H, Biomaterials., 24, 4385 (2003)

[10] Hern DL, Hubbell JA, J. Biomed. Mater. Res., 39, 266 (1998)

[11] Massia SP, Hubbell JA, J. Cell Biol., 114, 1089 (1991)

[12] Shin H, Jo S, Mikos AG, Biomaterials., 24, 4353 (2003)

[13] Benoit DSW, Schwartz MP, Durney AR, Anseth KS, Nat. Mater., 10, 816 (2008)

[14] Engler AJ, Sen S, Sweeney HL, Discher DE, Cell., 126, 677 (2006)

[15] Stevens MM, George JH, Science., 310, 1135 (2005)

[16] Pek YS, Wan ACA, Yang JY, Biomaterials., 31, 385 (2009)

[17] Choi BG, Park MH, Cho SH, Joo MK, Oh HJ, Kim EH, Park K, Han DK, Jeong B, Soft Matter., 7, 456 (2011)

[18] Kloxin AM, Kasko AM, Salinas CN, Anseth KS, Science., 324, 59 (2009)

[19] Lee H, Park TG, J. Biomed. Mater. Res. A., 88, 797 (2009)

[20] Joo MK, Park MH, Choi BG, Jeong B, J. Mater.Chem., 19, 5891 (2009)

[21] Robb SA, Lee BH, McLemore R, Vernon BL, Biomacromolecules, 8(7), 2294 (2007)

[22] Choi BG, Cho SH, Lee H, Cha MH, Park K, Jeong B, Han DK, Macromolecules, 44(7), 2269 (2011)

[23] Cha MH, Choi J, Choi BG, Park K, Kim IH, Jeong B, Han DK, J. Colloid Interface Sci., 360(1), 78 (2011)

[24] Mortensen K, Perdersen S, Macromolecules., 26, 805 (1993)

[25] Malmsten M, Lindman B, Macromolecules,, 25, 5446 (1992)

[26] Choi BG, Park MH, Cho SH, Joo MK, Oh HJ, Kim EH, Park K, Han DK, Jeong B, Biomaterials., 31, 9266 (2010)

[27] Mark K, Gauss V, Mark H, Muller P, Nature., 267, 531 (1977)

[28] Chung C, Erickson IE, Mauck RL, Burdick JA, Tissue Eng. Part A., 14, 1121 (2008)