A new electrochemical biosensor for hydrogen peroxide using HRP/AgNPs/cysteamine/p-ABSA/GCE self-assembly modified electrode

Ali Shokuhi Rad; Mohsen Jahanshahi; Mehdi Ardjmand; Ali-Akbar Safekordi

Korean Journal of Chemical Engineering, Vol.29, No.12, 1766-1770, December, 2012

DOI10.1007/s11814-012-0078-0 Export Citation

A new electrochemical biosensor for hydrogen peroxide using HRP/AgNPs/cysteamine/p-ABSA/GCE self-assembly modified electrode

Ali Shokuhi Rad^†, Mohsen Jahanshahi¹, Mehdi Ardjmand², and Ali-Akbar Safekordi³

Chemical Engineering Group, Faculty of Engineering, Qaemshahr Branch, Islamic Azad University, Tehran, Iran
¹Nanotechnology Research Institute, School of Chemical Engineering, Babol University of Technology, Babol, Iran
²Chemical Engineering Group, Faculty of Engineering, South Tehran Branch, Islamic Azad University, Tehran, Iran
³Chemical Engineering Group, Faculty of Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

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An electrochemical hydrogen peroxide biosensor was designed by immobilizing horseradish peroxidase (HRP) on Ag nanoparticles/cysteamine/p-aminobenzene sulfonic acid/glassy carbon (GC) electrode. Ag nanoparticles can act as tiny conduction centers on electrodes that adsorb redox enzymes, facilitating the transfer of electrons with no requiring any loss of biological activity. The forerunner film was first electropolymerized on the glassy carbon electrode with p-aminobenzene sulfonic acid (p-ABSA) by cyclic voltammetry. The cysteamine (CA) was bound on the surface of the film by electrostatic force, then Ag nanoparticles were immobilized on the cysteamine monolayer, and lastly HRP was adsorbed onto the surfaces of the Ag nanoparticles. A dramatic decrease in the overvoltage of H2O2 was observed with improved sensitivity, which makes the modified electrodes of great promise for oxidase-based amperometric biosensors. The biosensor responded to H2O2 in the linear range from 1.2×106 mol/L to 9.8×103 mol/L with a detection limit of 1.1×108 mol/L. Moreover, the obtained biosensor exhibited good accuracy and high sensitivity.

Keywords:AgNP;Hydrogen Peroxide;Cycteamamin;Biosensor;Cyclic Voltammetry

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[2] Sun JJ, Fang HQ, Cheng HY, Analyst., 123, 1365 (1998)

[3] Liu Y, Yuan R, Li QF, Chai YQ, Zhong X, Tang DP, South African Journal of Chemistry., 580, 4 (2005)

[4] Tatsum T, Okawa Y, Watanabe T, Anal. Chem., 61, 2352 (1989)

[5] Fan C, Wang H, Sun S, Zhu D, Wagner G, Li G, Anal. Chem., 73, 2850 (2001)

[6] Zhang JD, Oyama M, Electrochim. Acta, 50(1), 85 (2004)

[7] Shumyantseva VV, Ivanov YD, Bistolas NF, Scheller W, Archakov AI, Wollenberger U, Anal. Chem., 76, 6046 (2004)

[8] Dai ZH, Liu SQ, Ju HX, Electrochim. Acta, 49(13), 2139 (2004)

[9] Morrin A, Guzman A, Killard AJ, Pingarron JM, Smyth MR, Biosens. Bioelectron., 18, 715 (2003)

[10] Gao F, Yuan R, Chai Y, Tang M, Cao S, Chen S, Colloids Surf. A: Physicochem. Eng. Aspects., 295, 223 (2007)

[11] Chattopadhyay K, Mazurdar S, Bioelectrochemistry., 53, 17 (2001)

[12] Zhao S, Zhang K, Sun YY, Sun CQ, Bioelectrochemistry., 69, 10 (2006)

[13] Xu Y, Hu C, Hu S, Sensors and Actuators B., 130, 816 (2008)

[14] Liu CY, Hu JM, Biosens. Bioelectron., 24, 2149 (2009)

[15] Ren C, Song Y, Li Z, Zhu G, Anal. Bioanal. Chem., 381, 1179 (2005)

[16] Tang DP, Yuan R, Chai YQ, Zhong X, Liu Y, Dai J, Biochem.Eng. J., 22, 43 (2004)

[17] Cao YW, Jin R, Mirkin CA, J. Am. Chem. Soc., 123(32), 7961 (2001)

[18] Lai GS, Zhang HL, Han DY, Sens. Actuators B: Chem., 129, 497 (2008)

[19] Xu Q, Mao C, Liu NN, Zhu JJ, Shen J, React. Funct.Polym., 66, 863 (2006)

[20] Wang G, Wang W, Wu J, Liu H, Jiao S, Fang B, Microchim Acta., 164, 149 (2009)