| 초록 |
In recent years, the CO2 concentration was continually increase. Rhodobacter sphaeroides, one of non-sulfur purple photosynthetic bacteria, have an ability to CO2 fixation. The recombinant Escherichia coli harboring 11 genes of Calvin cycle using a dual plasmid system; having 5 genes in Form I operon and having 6 genes in Form II operon. The Calvin cycle proteins were successfully expression in E. coli and the CO2 reduction of recombinant E. coli transformed whole Calvin cycle was the highest. In addition, the increase of CO2 reduction efficiency by treating FBP, RuBP, and Mg2+. TEM image and intracellular ATP and NAD+/NADH ratio results indicated the increase of activity. The protein expressions existed Mg2+ was strongly expression than treated IPTG. The amount of residual CO2 in recombinant E. coli was lower than R. sphaeroides, however, per-cell base measurement was higher than R. sphaeroides using co-culture with S. cerevisiae. This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No: PJ01051502)” Rural Development Administration, Republic of Korea. |
