| 초록 |
It is widely known that a technology of hydrogen production using green algae can simultaneously overcome both problems of energy and environment. We performed study of hydrogen production using the green algal Chlamydomonas reinhardtii in the sulfur deprivation condition. To produce hydrogen by the green algae is required hydrogenase activation. But hydrogenase having severe O2 sensitivity are inactivated by photosynthetic O2 evolution. This problem can overcome to separate between the simultaneous photosynthetic O2 and H2 gas evolution (two-stage) by the sulfur deprivation in medium. Two-stage process was performed photosynthetic O2 evolution and carbon accumulation (stage 1), followed by H2 evolution consuming endogenous substrate when the green algae are incubated under anaerobic condition(stage 2). After 30 hours of sulfur deprivation, the O2 evolution rate by photosynthesis fell below the O2 uptake rate by respiration. In addition, we investigated effect of induction parameters such as cell density, cell growth stage, and light intensity on hydrogen productivity in C. reinhardtii.
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