| 초록 |
The hydrogen peroxide (H2O2) biosensor composed of the bismuth selenide nanoparticle (Bi2Se3 NP), gold (Au) and eight silver-ion mediated double-stranded DNA (mDNA) is fabricated for enhancement of electrochemical signal and stability. For the first time, the synthesized Bi2Se3 NP is immobilized uniformly on the gold electrode by self-assembly. Additionally, the Au layer is deposited on the Bi2Se3 NP layer to increase the stability of the electrochemical signal (Bi2Se3/Au). Then, to detect H2O2, the mDNA is immobilized on the Bi2Se3/Au layer by Au-thiol bonding (Bi2Se3/Au/mDNA). The immobilized Bi2Se3 induces the efficient electron transfer to the mDNA and Au electrode. To investigate H2O2 detection performance of the fabricated biosensor in real sample, the phorbol 12-myristate 13-acetate (PMA) is treated to the two breast cancer cell.The prepared biosensor successfully classifies two breast cancer cell through the amount of released H2O2. The fabricated biosensor provides the high electrochemical signal compared to the biosensor without Bi2Se3/Au. This proposed hybrid material can be used as the platform to develop biosensor for high selectivity and low detection limit. |
