메틸 바이올렛이 함유된 키토산 마이크로 캡슐의 제조와 그 방출특성

하병조; 이옥섭; 이윤식

Journal of the Korean Industrial and Engineering Chemistry, Vol.6, No.5, 951-958, October, 1995

Export Citation

메틸 바이올렛이 함유된 키토산 마이크로 캡슐의 제조와 그 방출특성

Preparation and Release Kinetics of Chitosan Mirocapsules Containing Methyl Violet

하병조, 이옥섭, 이윤식

초록

에멀젼 상분리법과 글루타르알데히드를 사용한 가교반응에 의해 메틸바이올렛이 함유된 키토산 마이크로캡슬을 제조하였다. 이들의 제조를 위해 먼저 수상/유화제/유기상으로 이루어진 W/O 에멀젼을 형성시킨 후 에멀젼의 안정도에 미치는 요소들에 대해 조사하였다. 유기상이 톨루엔인 경우 솔비탄 모노올레이트 (HLB=4.3)를 사용했을 때 상대적으로 높은 유화안정성을 나타내었다. 또한 보조계면활성제로 n-헥산을올 첨가해 줌으로써 유화안정성이 상승적으로 증가함이 관찰되었다. 제조된 마이크로캡슬의 내부를 전자현미경을 통해 관찰한 결과 약 8㎛ 정도의 표피층이 발견되었으며, 마이크로캡슬의 평균 입자크기는 약 250㎛이었다. 마이크로캡술의 pH변화에 따른 팽윤부피를 관찰한 결과 산성조건 (pH=3)일수록 높은 팽윤성을 나타내었으며, 알칼리조건 (pH=9)에서는 팽윤이 거의 일어나지 않음을 알 수 있었다. 또한 양성자성 용매가 비양성자성 용매에 비해 상대적으로 높은 괭윤성을 보였다. 한편 키토산 마이크로캡슬에 함유된 메틸바이올렛의 방출거동을 pH 변화 및 Iysozyme 존재하에서 관찰한 결과, pH 5.1의 초산염 완충용액에서 Iysozyme을 첨가한 경우 가장 높은 방출현상을 나타내었다.

Crosslinked-chitosan microcapsules containing methyl violet were prepared by emulsion phase separation method. The factors influencing the stability of W/O emulsions, composed of water phase/emulsifier/organic phase were examined. Sorbitan monooleate (HLB= 4.3) showed higher emulsion stability in water/toluene system. The addition of n-hexanol as cosurfactant synergistically increased the emulsion stability. A typical microcapsules had a mean diameter of ,250㎛ with a skin layer of 8㎛. The swelling volumes at different pH indicated that the degree of swelling increases in acidic condition, but not in alkaline condition. Also, the swelling volumes in protic solvents showed relatively higher than in aprotic solvents. Release of methyl violet from the microcapsules was also examined at different pH conditions with or without lysozyme. The results showed that the addition of lysozyme to the suspension medium at pH 5.1 acetate buffer gave the highest release rate of methyl violet.

[References]

Muzzarelli RAA, "Chitin," Pergamon Press, Oxford (1977)
Muzzarelli RAA, "Natural Chelating Polymer," Pergamon Press, New York (1973)
Gudmund SB, Thorleif A, Paul S, "Chitin and Chitosan," Elsevier Science Publishers, New York (1989)
Son SI, Chang BK, Choi KS, J. Korean Ind. Eng. Chem., 3(1), 156 (1992)
Lee YM, Desalination, 90, 277 (1993)
Cha WS, Kim SI, Lee DB, Na JW, J. Korean Ind. Eng. Chem., 6(1), 8 (1995)
Kim CH, Nam HG, Jung BO, Kim JJ, Choi KS, J. Korean Ind. Eng. Chem., 6(3), 464 (1995)
Kim CH, Lee JW, Jung BO, Chang BK, Choi KS, Kim JJ, J. Korean Ind. Eng. Chem., 6(1), 130 (1995)
Uragami T, Matsuda T, Okuno H, Miyata T, J. Membr. Sci., 88(2-3), 243 (1994)
Jin CW, Kim CH, Choi KS, J. Korean Ind. Eng. Chem., 6(3), 482 (1995)
Kim JH, Lee YM, Polymer, 34, 1952 (1993)
Hou WM, Miyazaki S, Takada M, Komai T, Chem. Pharm. Bull., 33, 3985 (1985)
Horton D, Just EK, Carbohydr. Res., 29, 173 (1973)
Kojima K, Yoshikuni M, Suzuki T, J. Appl. Polym. Sci., 24, 1587 (1979)
Pangburn SH, Trescony PV, Heller J, Biomaterials, 3, 105 (1982)
Pantalenone D, Yalpani M, Scollar M, Carbohydr. Res., 237, 325 (1992)
Muzzarelli RAA, Carbohydr. Polym., 20, 7 (1993)
Uchida Y, Kag. Kog., 42, 793 (1991)
Knapczyk J, Macara AB, Pawlik B, Int. J. Pharm., 80, 33 (1992)
Rutherford FA, Austin PR, Proc. Int. Conf. Chitin/Chitosan, 1st, 182 (1978)
Sannan T, Kurita K, Ogura K, Iwakuro Y, Polymer, 19, 458 (1978)
Mark H, Houwink R, J. Prakt. Chem., 157, 15 (1941)
Park JW, Choi K, Park KK, Bull. Korean Chem. Soc., 4, 68 (1983)
Faldt P, Bergenstahl B, Claesson PM, Colloids Surf. A: Physicochem. Eng. Asp., 71, 187 (1993)
Pangburn SH, Trescony PV, Heller J, Biomaterials, 3, 105 (1982)
Powning RF, Irzykiewicz H, Biochim. Biophys. Acta, 124, 218 (1966)

[Referenced By]

[1] Muzzarelli RAA, "Chitin," Pergamon Press, Oxford (1977)

[2] Muzzarelli RAA, "Natural Chelating Polymer," Pergamon Press, New York (1973)

[3] Gudmund SB, Thorleif A, Paul S, "Chitin and Chitosan," Elsevier Science Publishers, New York (1989)

[4] Son SI, Chang BK, Choi KS, J. Korean Ind. Eng. Chem., 3(1), 156 (1992)

[5] Lee YM, Desalination, 90, 277 (1993)

[6] Cha WS, Kim SI, Lee DB, Na JW, J. Korean Ind. Eng. Chem., 6(1), 8 (1995)

[7] Kim CH, Nam HG, Jung BO, Kim JJ, Choi KS, J. Korean Ind. Eng. Chem., 6(3), 464 (1995)

[8] Kim CH, Lee JW, Jung BO, Chang BK, Choi KS, Kim JJ, J. Korean Ind. Eng. Chem., 6(1), 130 (1995)

[9] Uragami T, Matsuda T, Okuno H, Miyata T, J. Membr. Sci., 88(2-3), 243 (1994)

[10] Jin CW, Kim CH, Choi KS, J. Korean Ind. Eng. Chem., 6(3), 482 (1995)

[11] Kim JH, Lee YM, Polymer, 34, 1952 (1993)

[12] Hou WM, Miyazaki S, Takada M, Komai T, Chem. Pharm. Bull., 33, 3985 (1985)

[13] Horton D, Just EK, Carbohydr. Res., 29, 173 (1973)

[14] Kojima K, Yoshikuni M, Suzuki T, J. Appl. Polym. Sci., 24, 1587 (1979)

[15] Pangburn SH, Trescony PV, Heller J, Biomaterials, 3, 105 (1982)

[16] Pantalenone D, Yalpani M, Scollar M, Carbohydr. Res., 237, 325 (1992)

[17] Muzzarelli RAA, Carbohydr. Polym., 20, 7 (1993)

[18] Uchida Y, Kag. Kog., 42, 793 (1991)

[19] Knapczyk J, Macara AB, Pawlik B, Int. J. Pharm., 80, 33 (1992)

[20] Rutherford FA, Austin PR, Proc. Int. Conf. Chitin/Chitosan, 1st, 182 (1978)

[21] Sannan T, Kurita K, Ogura K, Iwakuro Y, Polymer, 19, 458 (1978)

[22] Mark H, Houwink R, J. Prakt. Chem., 157, 15 (1941)

[23] Park JW, Choi K, Park KK, Bull. Korean Chem. Soc., 4, 68 (1983)

[24] Faldt P, Bergenstahl B, Claesson PM, Colloids Surf. A: Physicochem. Eng. Asp., 71, 187 (1993)

[25] Pangburn SH, Trescony PV, Heller J, Biomaterials, 3, 105 (1982)

[26] Powning RF, Irzykiewicz H, Biochim. Biophys. Acta, 124, 218 (1966)