Preparation of phosphonated gelatin-coated titanium containing rhBMP-2 by UV irradiation for improved osteoinduction and function

Yun Heo; Eun-Hye Kim; Eiry Kobatake; Jae-Woon Nah; Yoshihiro Ito; Tae-Il Son

Journal of Industrial and Engineering Chemistry, Vol.36, 66-73, April, 2016

DOI10.1016/j.jiec.2016.01.006 Export Citation

Preparation of phosphonated gelatin-coated titanium containing rhBMP-2 by UV irradiation for improved osteoinduction and function

Yun Heo, Eun-Hye Kim, Eiry Kobatake¹, Jae-Woon Nah², Yoshihiro Ito³, and Tae-Il Son^†

Department of Systems Biotechnology and Bio-Environmental Technology (BET) Research Institute, Chung-Ang University, Anseong-si 456-756, Gyeonggi-do, Republic of Korea
¹Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Midori-ku 226-8502, Yokohama, Japan
²Department of Polymer Science and Engineering, Sunchon National University, 255 Jungang-ro, Sunchon 540-950, Jeollanam-do, Republic of Korea
³Nano Medical Engineering Laboratory, RIKEN, Wako, Saitama 351-0198, Japan

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Gelatin derivative was prepared to coating titanium surface and photo-immobilize rhBMP-2 for improved osteoinduction and function. The conjugation was confirmed by 31P and 1H NMR. Photo-immobilization of the azide-phosphonate-gelatin (Az-P-Gel) was evaluated by micropatterning. The rate of recombinant human bone morphogenetic protein (rhBMP-2) release was evaluated for performance of Az-P-Gel as a carrier. The water contact angle was determined to characterize Ti surface coated with Az-P-Gel. Ability to induce osteogenesis of Az-P-Gel was evaluated using alkaline phosphatase (ALP) activity assay, Alizarin Red S staining, and calcium colorimetric assay. Our results demonstrate that Ti modified with Az-P-Gel and rhBMP-2 facilitates bone regeneration.

Keywords:Gelatin;Drug-delivery system;Osteoinduction;Titanium surface modification;Photo-immobilization

[References]

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Niinomi M, Mater. Sci. Eng. A-Struct. Mater. Prop. Microstruct. Process., 243, 231 (1998)
Miron RJ, Oates CJ, Molenberg A, Dard M, Hamilton DW, Biomaterials, 31, 449 (2010)
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Wang YM, Lei TQ, Jiang BL, Guo LX, Appl. Surf. Sci., 233(1-4), 258 (2004)
Xiao S, Textor M, Spencer ND, Wieland M, Keller B, Sigrist H, J. Mater. Sci. -Mater. Med., 8, 867 (1997)
Schuler M, Owen GR, Hamilton DW, de Wild M, Textor M, Brunette DM, Tosatti SG, Biomaterials, 27, 4003 (2006)
Kang SW, Kim JS, Park KS, Cha BH, Shim JH, Kim JY, Cho DW, Rhie JW, Lee SH, Bone, 48, 298 (2011)
Puleo D, Kissling R, Sheu MS, Biomaterials, 23, 2079 (2002)
Cheng H, Jiang W, Phillips FM, Haydon RC, Peng Y, Zhou L, Luu HH, An N, Breyer B, Vanichakarn P, J. Bone Joint Surg. -Br. Vol., 85, 1544 (2003)
Na K, Kim SW, Sun BK, Woo DG, Yang HN, Chung HM, Park KH, Biomaterials, 28, 2631 (2007)
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Kim J, Kim IS, Cho TH, Lee KB, Hwang SJ, Tae G, Noh I, Lee SH, Park Y, Sun K, Biomaterials, 28, 1830 (2007)
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Jeon O, Song SJ, Yang HS, Bhang SH, Kang SW, Sung MA, Lee JH, Kim BS, Biochem. Biophys. Res. Commun., 369(2), 774 (2008)
Chakfe N, Marois Y, Guidoin R, Deng X, Marois M, Roy R, King M, Douville Y, Polym. Polym. Compos., 1, 229 (1993)
Park SH, Seo SY, Lee HJ, Na HN, Lee JW, Woo HD, Son TI, Macromol. Res., 20(8), 842 (2012)
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Wang JS, Acta Orthop., 67, 1 (1996)
Park SH, Zhu L, Tada S, Obuse S, Yoshida Y, Nakamura M, Son TI, Tsuneda S, Ito Y, Polym. Int., 63, 1616 (2014)
Heo Y, Park SH, Seo SY, Yun JY, Ito Y, Son TI, Macromol. Res., 22(5), 541 (2014)
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Berglundh T, Persson L, Klinge B, J. Clin. Periodontol., 29, 197 (2002)
Buser D, Broggini N, Wieland M, Schenk R, Denzer A, Cochran D, Hoffmann B, Lussi A, Steinemann S, J. Dent. Res., 83, 529 (2004)
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Ito Y, Hasuda H, Sakuragi M, Tsuzuki S, Acta Biomater., 3, 1024 (2007)
Kim KI, Lee JW, Ito Y, Kang JH, Song KS, Jang EC, Son TI, J. Appl. Polym. Sci., 117(5), 3029 (2010)
Bancroft GN, Sikavitsas VI, van den Dolder J, Sheffield TL, Ambrose CG, Jansen JA, Mikos AG, Proc. Natl. Acad. Sci. U.S.A., 99, 12600 (2002)
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Park YJ, Kim KH, Lee JY, Ku Y, Lee SJ, Min BM, Chung CP, Biotechnol. Appl. Biochem., 43, 17 (2006)

[Referenced By]

[1] Okazaki Y, Rao S, Ito Y, Tateishi T, Biomaterials, 19, 1197 (1998)

[2] Niinomi M, Mater. Sci. Eng. A-Struct. Mater. Prop. Microstruct. Process., 243, 231 (1998)

[3] Miron RJ, Oates CJ, Molenberg A, Dard M, Hamilton DW, Biomaterials, 31, 449 (2010)

[4] Piskounova S, Forsgren J, Brohede U, Engqvist H, Stromme M, J. Biomed. Mater. Res. B: Appl. Biomater., 91, 780 (2009)

[5] Shi ZL, Neoh KG, Kang ET, Poh CK, Wang W, Biomacromolecules, 10(6), 1603 (2009)

[6] Oonishi H, Yamamoto M, Ishimaru H, Tsuji E, Kushitani S, Aono M, Ukon Y, J. Bone Joint Surg. -Br. Vol., 71, 213 (1989)

[7] Wang YM, Lei TQ, Jiang BL, Guo LX, Appl. Surf. Sci., 233(1-4), 258 (2004)

[8] Xiao S, Textor M, Spencer ND, Wieland M, Keller B, Sigrist H, J. Mater. Sci. -Mater. Med., 8, 867 (1997)

[9] Schuler M, Owen GR, Hamilton DW, de Wild M, Textor M, Brunette DM, Tosatti SG, Biomaterials, 27, 4003 (2006)

[10] Kang SW, Kim JS, Park KS, Cha BH, Shim JH, Kim JY, Cho DW, Rhie JW, Lee SH, Bone, 48, 298 (2011)

[11] Puleo D, Kissling R, Sheu MS, Biomaterials, 23, 2079 (2002)

[12] Cheng H, Jiang W, Phillips FM, Haydon RC, Peng Y, Zhou L, Luu HH, An N, Breyer B, Vanichakarn P, J. Bone Joint Surg. -Br. Vol., 85, 1544 (2003)

[13] Na K, Kim SW, Sun BK, Woo DG, Yang HN, Chung HM, Park KH, Biomaterials, 28, 2631 (2007)

[14] Sellers RS, Zhang R, Glasson SS, Kim HD, Peluso D, D’augusta DA, Beckwith K, Morris EA, J. Bone Joint Surg. -Br. Vol., 82, 151 (2000)

[15] Kim J, Kim IS, Cho TH, Lee KB, Hwang SJ, Tae G, Noh I, Lee SH, Park Y, Sun K, Biomaterials, 28, 1830 (2007)

[16] Nie H, Soh BW, Fu YC, Wang CH, Biotechnol. Bioeng., 99(1), 223 (2008)

[17] Jeon O, Song SJ, Yang HS, Bhang SH, Kang SW, Sung MA, Lee JH, Kim BS, Biochem. Biophys. Res. Commun., 369(2), 774 (2008)

[18] Chakfe N, Marois Y, Guidoin R, Deng X, Marois M, Roy R, King M, Douville Y, Polym. Polym. Compos., 1, 229 (1993)

[19] Park SH, Seo SY, Lee HJ, Na HN, Lee JW, Woo HD, Son TI, Macromol. Res., 20(8), 842 (2012)

[20] Sasisekharan R, Ernst S, Venkataraman G, Angiogenesis, 1, 45 (1997)

[21] Ishibe T, Goto T, Kodama T, Miyazaki T, Kobayashi S, Takahashi T, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endodontol., 108, 867 (2009)

[22] Perets A, Baruch Y, Weisbuch F, Shoshany G, Neufeld G, Cohen S, J. Biomed. Mater. Res. A, 65, 489 (2003)

[23] Yazdimamaghani M, Vashaee D, Assefa S, Walker K, Madihally S, Kohler G, Tayebi L, J. Biomed. Nanotechnol., 10, 911 (2014)

[24] VigaSlenters T, J. Mater. Chem., 18, 5359 (2008)

[25] Wang JS, Acta Orthop., 67, 1 (1996)

[26] Park SH, Zhu L, Tada S, Obuse S, Yoshida Y, Nakamura M, Son TI, Tsuneda S, Ito Y, Polym. Int., 63, 1616 (2014)

[27] Heo Y, Park SH, Seo SY, Yun JY, Ito Y, Son TI, Macromol. Res., 22(5), 541 (2014)

[28] Ito Y, Hasuda H, Yamauchi T, Komatsu N, Ikebuchi K, Biomaterials, 25, 2293 (2004)

[29] Heo Y, Lee HJ, Kim EH, Kim MK, Ito Y, Son TI, J. Appl. Polym. Sci., http://dx.doi.org/10.1002/app.40113, 40113 (1 of 10).40113 (10 of 10), 131 (2014)

[30] Kwon YD, Karbach J, Wagner W, Al-Nawas B, Clin. Oral Implants Res., 21, 316 (2010)

[31] Berglundh T, Persson L, Klinge B, J. Clin. Periodontol., 29, 197 (2002)

[32] Buser D, Broggini N, Wieland M, Schenk R, Denzer A, Cochran D, Hoffmann B, Lussi A, Steinemann S, J. Dent. Res., 83, 529 (2004)

[33] Le Guehennec L, Soueidan A, Layrolle P, Amouriq Y, Dent. Mater., 23, 844 (2007)

[34] Ito Y, Hasuda H, Sakuragi M, Tsuzuki S, Acta Biomater., 3, 1024 (2007)

[35] Kim KI, Lee JW, Ito Y, Kang JH, Song KS, Jang EC, Son TI, J. Appl. Polym. Sci., 117(5), 3029 (2010)

[36] Bancroft GN, Sikavitsas VI, van den Dolder J, Sheffield TL, Ambrose CG, Jansen JA, Mikos AG, Proc. Natl. Acad. Sci. U.S.A., 99, 12600 (2002)

[37] Lin H, Zhao Y, Sun W, Chen B, Zhang J, Zhao W, Xiao Z, Dai J, Biomaterials, 29, 1189 (2008)

[38] Park YJ, Kim KH, Lee JY, Ku Y, Lee SJ, Min BM, Chung CP, Biotechnol. Appl. Biochem., 43, 17 (2006)