Salt-Induced Protein Precipitation in Aqueous Solution: The Effect of Pre-Aggregation

Sang Gon Kim; Young Chan Bae; Si Ok Ryu

Korean Journal of Chemical Engineering, Vol.17, No.6, 638-642, November, 2000

DOI10.1007/BF02699110 Export Citation

Salt-Induced Protein Precipitation in Aqueous Solution: The Effect of Pre-Aggregation

Sang Gon Kim, Young Chan Bae^†, and Si Ok Ryu¹

Dept. of Industrial Chemistry and Molecular Thermodynamics Lab., Hanyang University, Seoul 133-791, Korea
¹School of Chemical Engineering and Technology, Yeungnam University, 214-1 Dae-Dong, Gyongsan 712-749, Korea

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A molecular-thermodynamic model is developed for the salt-induced protein precipitation The protein molecules interact through four intermolecular potentials. An equation of state is derived based on the statistical mechanical perturbation theory with the modified Chiew's equation as the reference system and a perturbation based on the protein-protein interaction potential of mean force. The equation of state provides an expression for the chemical potential of the protein and determines liquid-liquid equilibria. The precipitation behaviors are studied by calculating the partition coefficient with changing conditions such as ionic strength, protein and salt size, and the degree of pre-aggregation effect. The reasonable pre-aggregation effects are determined by comparing the proposed model with experimental data.

Keywords:Protein;Interaction Potentials;Precipitation;Phase Equilibria;Pre-aggregation

[References]

Albertsson PA, "Partition of Cell Particles and Macromolecules," Wiley, New York (1986)
Chiew YC, Mol. Phys., 70, 129 (1990)
Cho D, Cornec M, Korean J. Chem. Eng., 16(3), 371 (1999)
Coen CJ, Blanch HW, Prausnitz JM, AIChE J., 41(4), 996 (1995)
Cohn EJ, Edsall JT, "Proteins, Amino Acids, and Peptides," Reinhold, New York (1943)
Dixon M, Webb EC, Adv. Protein Chem., 16, 199 (1961)
Foster PR, Dunhill P, Lilly MD, Biochem. Biophys. Acta, 317, 505 (1975)
Haire RN, Tisel WA, White JC, Rosenberg A, Biopolymers, 23, 2761 (1984)
Hong DP, Kuboi R, Komasawa I, Korean J. Chem. Eng., 14(5), 334 (1997)
Isrelachvlili JN, "Intermolecular and Surface Forces," Academic Press, London (1985)
Kuehner DE, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 116(1-2), 140 (1996)
Kuehner D, Heyer C, Ramsch C, Fornefeld UM, Blanch HW, Biophysical J., 73, 3211 (1997)
Lee SH, Kim KR, Shon JS, Yoo JH, Chung H, Korean J. Chem. Eng., 16(2), 166 (1999)
Lee WK, Bani-Jaber A, McGuire J, Daeschel MA, Jung IH, Korean J. Chem. Eng., 17(2), 179 (2000)
Mahadevan H, Hall CK, AIChE J., 36, 1517 (1990)
Mahadevan H, Hall CK, AIChE J., 38, 573 (1992)
Niederauer MQ, Glatz CE, Adv. Biochem. Eng. Technol., 47, 159 (1992)
Park TH, Korean J. Chem. Eng., 11(4), 282 (1994)
Prausnitz JM, Lichtenthaler RN, Azevedo EGD, "Molecular Thermodynamics of Fluid Phase Equilibria," Prentice-Hall, Englewood Cliffs, NJ (1986)
Rothstein F, "Protein Precipitation Process Engineering," R.G. Harrion, ed., Dekker, New York (1994)
Seo KW, Oh JK, Korean J. Chem. Eng., 17(1), 47 (2000)
Shih YC, Blanch HW, Prausnitz JM, Biotechnol. Bioeng., 40, 1155 (1992)
Song YH, Lambert SM, Prausnitz JM, Ind. Eng. Chem. Res., 33(4), 1047 (1994)
Tanford C, "The Hydrophobic Effect: Formation of Micelles and Biological Membranes," Wiley, New York (1980)
Valchy V, Blanch HW, Prausnitz JM, AIChE J., 39, 215 (1993)
Valchy V, Blanch HW, Prausnitz JM, AIChE J., 39, 215 (1993)
Verwey E, Overbeek J, "Theory of Stability of Lyophobic Colloids," Elsevier, Amsterdam (1948)
Yoo KP, Shin HY, Noh MJ, You SS, Korean J. Chem. Eng., 14(5), 341 (1997)

[Referenced By]

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[1] Albertsson PA, "Partition of Cell Particles and Macromolecules," Wiley, New York (1986)

[2] Chiew YC, Mol. Phys., 70, 129 (1990)

[3] Cho D, Cornec M, Korean J. Chem. Eng., 16(3), 371 (1999)

[4] Coen CJ, Blanch HW, Prausnitz JM, AIChE J., 41(4), 996 (1995)

[5] Cohn EJ, Edsall JT, "Proteins, Amino Acids, and Peptides," Reinhold, New York (1943)

[6] Dixon M, Webb EC, Adv. Protein Chem., 16, 199 (1961)

[7] Foster PR, Dunhill P, Lilly MD, Biochem. Biophys. Acta, 317, 505 (1975)

[8] Haire RN, Tisel WA, White JC, Rosenberg A, Biopolymers, 23, 2761 (1984)

[9] Hong DP, Kuboi R, Komasawa I, Korean J. Chem. Eng., 14(5), 334 (1997)

[10] Isrelachvlili JN, "Intermolecular and Surface Forces," Academic Press, London (1985)

[11] Kuehner DE, Blanch HW, Prausnitz JM, Fluid Phase Equilib., 116(1-2), 140 (1996)

[12] Kuehner D, Heyer C, Ramsch C, Fornefeld UM, Blanch HW, Biophysical J., 73, 3211 (1997)

[13] Lee SH, Kim KR, Shon JS, Yoo JH, Chung H, Korean J. Chem. Eng., 16(2), 166 (1999)

[14] Lee WK, Bani-Jaber A, McGuire J, Daeschel MA, Jung IH, Korean J. Chem. Eng., 17(2), 179 (2000)

[15] Mahadevan H, Hall CK, AIChE J., 36, 1517 (1990)

[16] Mahadevan H, Hall CK, AIChE J., 38, 573 (1992)

[17] Niederauer MQ, Glatz CE, Adv. Biochem. Eng. Technol., 47, 159 (1992)

[18] Park TH, Korean J. Chem. Eng., 11(4), 282 (1994)

[19] Prausnitz JM, Lichtenthaler RN, Azevedo EGD, "Molecular Thermodynamics of Fluid Phase Equilibria," Prentice-Hall, Englewood Cliffs, NJ (1986)

[20] Rothstein F, "Protein Precipitation Process Engineering," R.G. Harrion, ed., Dekker, New York (1994)

[21] Seo KW, Oh JK, Korean J. Chem. Eng., 17(1), 47 (2000)

[22] Shih YC, Blanch HW, Prausnitz JM, Biotechnol. Bioeng., 40, 1155 (1992)

[23] Song YH, Lambert SM, Prausnitz JM, Ind. Eng. Chem. Res., 33(4), 1047 (1994)

[24] Tanford C, "The Hydrophobic Effect: Formation of Micelles and Biological Membranes," Wiley, New York (1980)

[25] Valchy V, Blanch HW, Prausnitz JM, AIChE J., 39, 215 (1993)

[26] Valchy V, Blanch HW, Prausnitz JM, AIChE J., 39, 215 (1993)

[27] Verwey E, Overbeek J, "Theory of Stability of Lyophobic Colloids," Elsevier, Amsterdam (1948)

[28] Yoo KP, Shin HY, Noh MJ, You SS, Korean J. Chem. Eng., 14(5), 341 (1997)