HWAHAK KONGHAK, Vol.32, No.3, 441-447, June, 1994
알코올 첨가 탄소원에 의한 PHB 및 P(HB-co-HV)의 생합성에 관한 연구
The Effects of Alcohols in Substrate on the Biosynthesis of PHB and P(HB-co-HV)
초록
본 연구에서는 상업화 균주인 Alcaligenes eutrophus NCIB 11599를 이용하여 글루코오스와 비교적 저가의 새로운 탄소원인 알코올을 사용, PHB 및 P(HB-co-HV)의 합성과 그 물성에 관하여 연구하였다. 알코올은 methanol, ethanol, propanol, butanol 및 pentanol 등을 사용하였다. 0.4g/L이하의 알코올을 10g/L 글루코오스와 함께 탄소원기질로 사용한 경우, 균의 비증식속도와 고분자의 축적율이 향상되었다. 비증식속도에 있어서는 ethanol을 첨가한 경우 최고 12%, pentanol의 경우 최고 20%, 그리고 고분장의 축적율에 있어서는 ethanol의 경우, 최고 40%, pentanol의 경우 최고 36%의 향상을 보였다. Methanol을 제외한 홀수개의 탄소수를 가진 알코올(propanol, pentanol)을 글루코오스와 함께 기질로 사용한 경우 P(HB-co-HV) 공중합체를 얻을 수 있었으며, 공급탄소원중의 알코올의 함량이 증가할수록 공중합체내의 HV함량이 증가하였다. Propanol과 pentanol을 첨가하여 합성한 공중합체의 융점은 기질중의 알코올 함량이 증가할수록 감소하여 propanol을 사용한 경우 142℃까지 떨어졌다. 한편 그 이외의 알코올(methanol, ethanol, butanol)을 사용할 경우에는 합성된 고분자의 융점이 알코올의 함량에는 무관하게 175℃로 일정하였다.
This study is concerned with the synthesis and the properties of PHB and P (HB-co-HV) copolymer in Alcaligenes eutrophus NCIB 11599 using glucose and alcohol as carbon sources. Alcohols such as methanol, ethanol, propanol, butanol and pentanol were used as carbon sources. When less than 0.4g/L alcohol with 10g/L glucose was added as a carbon source with glucose, the specific growth rate and the polymer accumulation rate were improved compared with using only glucose as a carbon source. The specific growth rate was increased to a maximum of 12% and 20% in ethanol and pentanol added substrate respectively. The polymer accumulation rate was increased to a maximum of 40% and 36% in ethanol and pentanol added substrate. P(HB-co-HV) copolymer was obtained when propanol or pentanol was used as a substrate with glucose. The HV fraction in copolymer was increased according to the increase of alcohol concentration in substrate. The melting temperature of copolymer synthesized by propanol or pentanol was decreased according to the increase of alcohol concentration. When propanol was used as a substrate, the melting temperature of copolymer was decreased to 142℃. On the other hand, the melting temperature of polymer synthesized by methanol, ethanol or butanol added substrate was constant at 175℃, the melting temperature of PHB.
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