화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Chemical Engineering Research, Vol.54, No.1, 89-93, February, 2016
DOI10.9713/kcer.2016.54.1.89  Export Citation
식물세포배양으로부터 파클리탁셀 및 이의 반합성 전구체 10-디아세틸파클리탁셀의 분리 양상
Separation Behavior of Paclitaxel and Its Semi-synthetic Precursor 10-Deacetylpaclitaxel from Plant Cell Cultures
이충기, 김진현†
  • 공주대학교 화학공학부, 31080 충남 천안시 서북구 천안대로 1223-24
Chung-Gi Lee, and Jin-Hyun Kim†
  • Department of Chemical Engineering, Kongju National University, 1223-24, Cheonan-daero, Seobuk-gu, Cheonan, Chungnam, 31080, Korea
E-mail:
초록
본 연구에서는 식물세포배양으로부터 항암물질 파클리탁셀 및 이의 반합성 전구체 10-디아세틸파클리탁셀의 분리양상을 조사하였다. 식물세포인 바이오매스를 이용한 분리/정제 공정인 용매를 이용한 바이오매스 추출, 액-액 추출, 흡착제 처리, 헥산 침전, 분별 침전을 순차적으로 수행한 결과, 흡착제 처리 공정에서 10-디아세틸파클리탁셀는 파클리탁셀로부터 가장 효과적으로 분리됨을 알 수 있었다. 파클리탁셀 및 10-디아세틸파클리탁셀 분리에 적합한 흡착제 종류, 건조시료/흡착제 비율, 흡착제 처리 온도는 각각 실로퓨트, 1:1.5(w/w), 20 oC 이었다. 최적의 흡착제 처리 조건에서 10-디아세틸파클리탁셀은 74.1% 분리/회수 가능하였다.
In this study, we investigated the separation behavior of the anticancer agent paclitaxel and its semi-synthetic precursor 10-deacetylpaclitaxel (10-DAP) from plant cell cultures. As a result of sequential separation/purification performed by biomass extraction with solvent, liquid-liquid extraction, adsorbent treatment, hexane precipitation, and fractional precipitation, the adsorbent treatment was found to be the most effective in separating and recovering 10-DAP from paclitaxel. The optimal adsorbent type, crude extract/adsorbent ratio, and adsorbent treatment temperature were sylopute, 1:1.5 (w/w), and 20 oC, respectively. The separation/recovery of 10-DAP from paclitaxel was 74.1% in adsorbent treatment process under optimal conditions.
Keywords:Paclitaxel;10-Deacetylpaclitaxel;Separation;Adsorbent Treatment;Optimization
  1. Kim GJ, Kim JH, Korean J. Chem. Eng., 32(6), 1023 (2015)
  2. Rao KV, Hanuman B, Alvarez C, Stoy M, Juchum J, Davies RM, Baxley R, Pharm. Res., 12, 1003 (1995)
  3. Baloglu E, Kingston DG, J. Nat. Prod., 62, 1068 (1999)
  4. Choi HK, Adams TL, Stahlhut RW, Kim SI, Yun JH, Song BK, Kim JH, Hong SS, Lee HS, “Method for Mass Production of Taxol by Semi-continuous Culture with Taxus chinensis Cell Culture,” U.S. Patent No. 5,871,979(1999).
  5. Park GY, Kim GJ, Kim JH, J. Ind. Eng. Chem., 21, 151 (2015)
  6. Kim JH, Gi US, Min BC, Hong SS, Lee HS, Biotechnol. Bioeng., 15, 398 (2000)
  7. Kang SH, Kim JH, Mun S, Process Biochem., 45, 1468 (2010)
  8. Ringel I, Horwitz SB, Susan BH, J. Pharmacol. Exp. Ther., 242, 692 (1987)
  9. Pyo SH, Choi HJ, Han BH, J. Chromatogr. A, 1123, 15 (2006)
  10. Kim JH, Kang IS, Choi HK, Hong SS, Lee HS, Process Biochem., 37, 679 (2002)
  11. Pyo SH, Park HB, Song BK, Han BH, Kim JH, Process Biochem., 39, 1985 (2004)
  12. Kim JH, Biotechnol. Bioeng., 21, 1 (2006)
  13. Gamborg OL, Miller RA, Ojima K, Exp. Cell Res., 50, 151 (1968)
  14. Choi HK, Son SJ, Na GH, Hong SS, Park YS, Song JY, Korean J. Plant Biotechnol., 29, 59 (2002)
  15. Lee CG, Kim JH, Korean Chem. Eng. Res., 52(4), 497 (2014)
  16. Lee CG, Kim JH, Process Biochem., 49, 1370 (2014)
  17. Pyo SH, Song BK, Ju CH, Han BH, Choi HJ, Process Biochem., 40, 1113 (2005)